Plasmid_Backbone

Part:BBa_K1677201:Design

Designed by: Mackenzie Labine-Romain   Group: iGEM15_BABS_UNSW_Australia   (2015-08-21)


pCPC


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

Primers containing biobrick prefix and suffix as well and other described parts were used to make this backbone RFC10 compatible.



Source

Plasmid was purchased from open source plasmid repository Addgene.com (originally named cpc-PHLS (g)). It was modified to be biobrick compatible. Formighieri, C., & Melis, A. (2014). Regulation of β-phellandrene synthase gene expression, recombinant protein accumulation, and monoterpene hydrocarbons production in Synechocystis transformants. Planta, 240(2), 309-324.

References